大理大学学报 ›› 2021, Vol. 6 ›› Issue (2): 39-45.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 02. 008

• 药学 • 上一篇    下一篇

基于网络药理学探讨猫爪草治疗肺结核的作用机制

刘向芳,王璐,陈洁,孙辉,陆绍玉,刘幸*   

  1. (昆明市第三人民医院药学部,昆明650041)
  • 收稿日期:2020-05-25 修回日期:2020-07-12 出版日期:2021-02-15 发布日期:2021-03-17
  • 通讯作者: 刘幸,副主任药师,E-mail:wsk2@163.com。
  • 作者简介:刘向芳,主管药师,主要从事医院药学研究。
  • 基金资助:
    昆明市科技计划重点项目(2017-1-S-1433)

Study on the Mechanism of Ranunculus ternatus in the Treatment of Pulmonary Tuberculosis Based on#br# Network Pharmacology

Liu Xiangfang, Wang Lu, Chen Jie, Sun Hui, Lu Shaoyu, Liu Xing*   

  1. (Department of Pharmacy, Kunming Third People´s Hospital, Kunming 650041, China)
  • Received:2020-05-25 Revised:2020-07-12 Online:2021-02-15 Published:2021-03-17

摘要: 目的:探讨猫爪草治疗肺结核潜在作用机制。方法:通过TCMSP数据库预测并筛选猫爪草的活性成分及其作用靶
点,利用Cytoscape 3.6.1软件构建猫爪草治疗肺结核活性成分和作用靶点的网络图,采用STRING 数据库构建蛋白互作网
络,利用Bioconductor数据分析靶点的GO功能和KEGG信号通路。结果:筛选得到12个活性成分及14个潜在的治疗靶点,通
过KEGG分析获得可能与肺结核相关的信号通路:细胞凋亡、p53信号通路、IL-17信号通路,对应基因为BCL2、BAX、CASP9、
CASP3、CASP8、JUN。结论:猫爪草中的活性成分可能通过细胞凋亡、p53 信号通路及IL-17 信号通路作用于BCL2、BAX、
CASP9 等靶点,进而发挥对肺结核的治疗作用,呈现多成分、多靶点和多通路的特点。

关键词: 猫爪草, 肺结核, 网络药理学, 靶基因, 信号通路

Abstract: Objective: To explore the potential mechanism of Ranunculus ternatus in the treatment of pulmonary tuberculosis.
Methods: The active components and targets of Ranunculus ternatus were predicted and screened by TCMSP database, Cytoscape 3.6.1
software was used to construct the network diagram of active components and targets in the treatment of pulmonary tuberculosis, using
STRING database to build PPI network. Bioconductor data were used to analyze the GO function and KEGG signaling pathway of the
target. Results: 12 active components and 14 potential therapeutic targets were screened, signaling pathways that may be associated
with tuberculosis were obtained by KEGG analysis: Apoptosis, p53 signaling pathway, and IL-17 signaling pathway; and corresponding
genes are BCL2, BAX, CASP9, CASP3, CASP8, and JUN. Conclusion: The active components of Ranunculus ternatus may act on
BCL2, BAX, CASP9 and other targets through apoptosis, p53 signaling pathway and IL-17 signaling pathway, and it is characterized by
multiple components, multiple targets and multiple pathways.

Key words: Ranunculus ternatus, pulmonary tuberculosis, network pharmacology, target gene, signal pathway

中图分类号: